Ancestry Research in Portugal
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 | Charles V of Austria, Holy Roman Emperor  (1500-1558)
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 | Isabella of Portugal, Holy Roman Empress (1503-1539)
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 | Philip II of Spain, King of Spain (1527-1598)
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| Anna of Austria (1549-1580)
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 | Ferdinand of Spain, Prince of Asturias (1571-1578) |
 | Charles Laurence of Spain (1573-1575) |
| Diego Félix of Spain (1575-1582) |
 | Philip III of Spain (1578-1621)  |
| Maria of Spain (1580-1583) |
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