Ancestry Research in Portugal
 MaleCharles V of Austria, Holy Roman Emperor |
| Birth | 24 Feb 1500 | |
| Death | 21 Sep 1558 | |
| Burial | ||
| Marriage | ||
| Father | Philip I of Austria | F274 Group Sheet | |
| Mother | Joanna of Castile, Queen of Castile and Aragon | F274 Group Sheet | |
 FemaleIsabella of Portugal, Holy Roman Empress |
| Birth | 24 Oct 1503 | Lisbon, Lisboa, Portugal  |
| Death | 1 May 1539 | Toledo, Castilla-La Mancha, Spain |
| Burial | ||
| Father | Manuel I of Portugal, King of Portugal | F257 Group Sheet | |
| Mother | Maria of Aragon, Queen of Portugal | F257 Group Sheet | |
 Male+ Philip II of Spain, King of Spain |
| Birth | 21 May 1527 | Palacio de Pimentel, Valladolid, Castilla-Leon, Spain |
| Death | 13 Sep 1598 | El Escorial, San Lorenzo de El Escorial, Madrid, Spain |
| Burial | ||
| Spouse | Maria Manuela of Portugal, Princess of Asturias | F260 | |
| Marriage | 12 Nov 1543 | Salamanca, Castilla-Leon, Spain |
| Spouse | Mary I of England | F637 | |
| Marriage | 25 Jul 1554 | Winchester Cathedral, Winchester, Hampshire, England |
| Spouse | Elisabeth of Valois | F641 | |
| Marriage | 1559 | Guadalajara, Castilla-La Mancha, Spain |
| Spouse | Anna of Austria | F635 | |
| Marriage | 1570 | |
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